منابع مشابه
DNA brick crystals with prescribed depths.
The ability to assemble functional materials with precise spatial arrangements is important for applications ranging from protein crystallography to photovoltaics. Here, we describe a general framework for constructing two-dimensional crystals with prescribed depths and sophisticated three-dimensional features. The crystals are self-assembled from single-stranded DNA components called DNA brick...
متن کاملDNA Brick Crystals with Prescribed Depth
We describe a general framework for constructing two-dimensional crystals with prescribed depth and sophisticated three-dimensional features. These crystals may serve as scaffolds for the precise spatial arrangements of functional materials for diverse applications. The crystals are selfassembled from single-stranded DNA components called DNA bricks. We demonstrate the experimental construction...
متن کاملDNA brick self-assembly with an off-lattice potential.
We report Monte Carlo simulations of a simple off-lattice patchy-particle model for DNA 'bricks'. We relate the parameters that characterise this model with the binding free energy of pairs of single-stranded DNA molecules. We verify that an off-lattice potential parameterised in this way reproduces much of the behaviour seen with a simpler lattice model we introduced previously, although the r...
متن کاملNumerical evidence for nucleated self-assembly of DNA brick structures.
The observation by Ke et al. [Science 338, 1177 (2012)] that large numbers of short, predesigned DNA strands can assemble into three-dimensional target structures came as a great surprise, as no colloidal self-assembling system has ever achieved the same degree of complexity. That failure seemed easy to rationalize: the larger the number of distinct building blocks, the higher the expected erro...
متن کاملProtocols for C-Brick DNA Standard Assembly Using Cpf1.
CRISPR-associated protein Cpf1 cleaves double-stranded DNA under the guidance of CRISPR RNA (crRNA), generating sticky ends. Because of this characteristic, Cpf1 has been used for the establishment of a DNA assembly standard called C-Brick, which has the advantage of long recognition sites and short scars. On a standard C-Brick vector, there are four Cpf1 recognition sites - the prefix (T1 and ...
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ژورنال
عنوان ژورنال: Nature Chemistry
سال: 2014
ISSN: 1755-4330,1755-4349
DOI: 10.1038/nchem.2083